Annotations of complete genome sequences submitted directly by sequencing projects are diverse in terms of annotation strategies and update frequencies, and these inconsistencies make comparative study difficult. To allow rapid data preparation of a large number of complete genomes, automation and speed are important objectives for genome re-annotation. Here we introduce an open-source rapid genome re-annotation software system, Restauro-G, specialized for bacterial genomes. Restauro-G re-annotates the genome by similarity searches utilizing the BLAST-Like Alignment Tool, referring to protein databases such as UniProt Knowledgebase, NCBI nr, NCBI COGs, PFAM, and Psortdb. Re-annotation by Restauro-G achieved over 98% accuracy for most bacterial chromosomes in comparison with the original manually curated annotation of EMBL releases. Restauro-G was developed in the generic bioinformatics workbench G-language Genome Analysis Environment and is distributed at http://restauro-g.iab.keio.ac.jp/ under the GNU General Public License.
Our manuscript describing this online resource is published by
Genomics Proteomics Bioinformatics journal with the title,
"Restauro-G: A Rapid Genome Re-Annotation System for Comparative Genomics" Satoshi Tamaki, Kazuharu Arakawa*, Nobuaki Kono, and Masaru
Please follow this link to the publisher site.
Genomes annotated by Restaruro-G
375 annotated genomes by Restauro-G are available from this page.
(C) 2006 Institute for Advanced Biosciences, Keio University